protein synthesis inhibitor chx Search Results


protein synthesis inhibitor chx  (Cayman Chemical)
90
Cayman Chemical protein synthesis inhibitor chx
IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the <t>E1</t> <t>inhibitor</t> MLN7243 (MLN, 5 μM) with <t>CHX</t> (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.
Protein Synthesis Inhibitor Chx, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+synthesis+inhibitor+chx/pmc09747586-219-15-19?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
protein synthesis inhibitor chx - by Bioz Stars, 2026-08
90/100 stars
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protein synthesis inhibitor chx  (J&K Scientific)
86
J&K Scientific protein synthesis inhibitor chx
IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the <t>E1</t> <t>inhibitor</t> MLN7243 (MLN, 5 μM) with <t>CHX</t> (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.
Protein Synthesis Inhibitor Chx, supplied by J&K Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+synthesis+inhibitor+chx/pm41286308-101-1-5?v=J%26K+Scientific
Average 86 stars, based on 1 article reviews
protein synthesis inhibitor chx - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier

Image Search Results


IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the E1 inhibitor MLN7243 (MLN, 5 μM) with CHX (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.

Journal: The Journal of Biological Chemistry

Article Title: The E3 ligase subunit FBXO45 binds the interferon-λ receptor and promotes its degradation during influenza virus infection

doi: 10.1016/j.jbc.2022.102698

Figure Lengend Snippet: IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the E1 inhibitor MLN7243 (MLN, 5 μM) with CHX (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.

Article Snippet: Twenty four to forty eight hours after transfection, the cells were then treated with the protein synthesis inhibitor CHX (Cayman Chemical, 40–50 μg/ml) for the indicated times before collection.

Techniques: Transfection, Ubiquitin Proteomics, Plasmid Preparation, Western Blot