protein synthesis inhibitor chx Search Results


protein synthesis inhibitor cycloheximide chx  (Fluka Chemical)
90
Fluka Chemical protein synthesis inhibitor cycloheximide chx
Protein Synthesis Inhibitor Cycloheximide Chx, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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de novo protein synthesis inhibitor chx  (Novoprotein)
90
Novoprotein de novo protein synthesis inhibitor chx
( A ) Hif1 α mRNA levels were examined in NRCM. n = 6/group. ( B ) After <t>CHX</t> treatment during reoxygenation, the <t>remaining</t> <t>Hif1α</t> protein levels in NRCM were examined by immunoblotting. n = 4/group. ( C ) After MG132 treatment during reoxygenation, the cumulative Hif1α protein levels in H/R NRCM were examined by immunoblotting. n = 4/group. Data are shown as mean ± SD. *** P < 0.001, ** P < 0.01, and * P < 0.05 by 2-way ANOVA followed by post hoc test.
De Novo Protein Synthesis Inhibitor Chx, supplied by Novoprotein, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein synthesis inhibitor cycloheximide  (Beyotime)
90
Beyotime protein synthesis inhibitor cycloheximide
( A ) Hif1 α mRNA levels were examined in NRCM. n = 6/group. ( B ) After <t>CHX</t> treatment during reoxygenation, the <t>remaining</t> <t>Hif1α</t> protein levels in NRCM were examined by immunoblotting. n = 4/group. ( C ) After MG132 treatment during reoxygenation, the cumulative Hif1α protein levels in H/R NRCM were examined by immunoblotting. n = 4/group. Data are shown as mean ± SD. *** P < 0.001, ** P < 0.01, and * P < 0.05 by 2-way ANOVA followed by post hoc test.
Protein Synthesis Inhibitor Cycloheximide, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein synthesis inhibitor cycloheximide/product/Beyotime
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protein synthesis inhibitor cycloheximide - by Bioz Stars, 2026-03
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protein synthesis inhibitor chx  (Bioscientifica Ltd)
90
Bioscientifica Ltd protein synthesis inhibitor chx
( A ) Hif1 α mRNA levels were examined in NRCM. n = 6/group. ( B ) After <t>CHX</t> treatment during reoxygenation, the <t>remaining</t> <t>Hif1α</t> protein levels in NRCM were examined by immunoblotting. n = 4/group. ( C ) After MG132 treatment during reoxygenation, the cumulative Hif1α protein levels in H/R NRCM were examined by immunoblotting. n = 4/group. Data are shown as mean ± SD. *** P < 0.001, ** P < 0.01, and * P < 0.05 by 2-way ANOVA followed by post hoc test.
Protein Synthesis Inhibitor Chx, supplied by Bioscientifica Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein synthesis inhibitor chx  (Cayman Chemical)
90
Cayman Chemical protein synthesis inhibitor chx
IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the <t>E1</t> <t>inhibitor</t> MLN7243 (MLN, 5 μM) with <t>CHX</t> (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.
Protein Synthesis Inhibitor Chx, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein synthesis inhibitor chx  (Beyotime)
90
Beyotime protein synthesis inhibitor chx
IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the <t>E1</t> <t>inhibitor</t> MLN7243 (MLN, 5 μM) with <t>CHX</t> (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.
Protein Synthesis Inhibitor Chx, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein synthesis inhibitor chx/product/Beyotime
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protein synthesis inhibitor chx - by Bioz Stars, 2026-03
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protein synthesis inhibitor cycloheximide chx 97064-72  (Amresco)
90
Amresco protein synthesis inhibitor cycloheximide chx 97064-72
IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the <t>E1</t> <t>inhibitor</t> MLN7243 (MLN, 5 μM) with <t>CHX</t> (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.
Protein Synthesis Inhibitor Cycloheximide Chx 97064 72, supplied by Amresco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein synthesis inhibitor cycloheximide chx 97064-72/product/Amresco
Average 90 stars, based on 1 article reviews
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( A ) Hif1 α mRNA levels were examined in NRCM. n = 6/group. ( B ) After CHX treatment during reoxygenation, the remaining Hif1α protein levels in NRCM were examined by immunoblotting. n = 4/group. ( C ) After MG132 treatment during reoxygenation, the cumulative Hif1α protein levels in H/R NRCM were examined by immunoblotting. n = 4/group. Data are shown as mean ± SD. *** P < 0.001, ** P < 0.01, and * P < 0.05 by 2-way ANOVA followed by post hoc test.

Journal: JCI Insight

Article Title: HSPA12A maintains aerobic glycolytic homeostasis and Histone3 lactylation in cardiomyocytes to attenuate myocardial ischemia/reperfusion injury

doi: 10.1172/jci.insight.169125

Figure Lengend Snippet: ( A ) Hif1 α mRNA levels were examined in NRCM. n = 6/group. ( B ) After CHX treatment during reoxygenation, the remaining Hif1α protein levels in NRCM were examined by immunoblotting. n = 4/group. ( C ) After MG132 treatment during reoxygenation, the cumulative Hif1α protein levels in H/R NRCM were examined by immunoblotting. n = 4/group. Data are shown as mean ± SD. *** P < 0.001, ** P < 0.01, and * P < 0.05 by 2-way ANOVA followed by post hoc test.

Article Snippet: To investigate whether HSPA12A regulates Hif1α stability by modulating its proteasomal degradation, the de novo protein synthesis inhibitor CHX (20 μM) and proteasome inhibitor MG132 (15 μM) was added to the NRCM culture for the indicated durations.

Techniques: Western Blot

IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the E1 inhibitor MLN7243 (MLN, 5 μM) with CHX (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.

Journal: The Journal of Biological Chemistry

Article Title: The E3 ligase subunit FBXO45 binds the interferon-λ receptor and promotes its degradation during influenza virus infection

doi: 10.1016/j.jbc.2022.102698

Figure Lengend Snippet: IFNLR1 is polyubiquitinated and proteasomally degraded. A , HEK-293T cells were transfected with plasmids encoding V5- and HIS-tagged IFNLR1 and/or HA-tagged ubiquitin. Twenty four hours later, cells were treated with DMSO, MG-132 (20 μM), bafilomycin A1 (BafA1, 100 nM), or MLN (5 μM) for 4 h. Cell lysates were harvested, and the HIS-tagged IFNLR1 receptor was pulled down using magnetic cobalt beads and eluted with imidazole. Pull-down (above) and whole cell lysates (below) were immunoblotted to determine polyubiquitinated IFNLR1 ( n = 2). B and C , HEK-293T cells were transfected with V5-tagged IFNLR1 plasmid for 24 h followed by pretreatment with DMSO, MG-132 (MG, 20 μM), or bafilomycin A1 (BafA1, 100 nM) or DMSO ( B ) or the E1 inhibitor MLN7243 (MLN, 5 μM) with CHX (50 μg/ml) at indicated time points. Shown graphically below are densitometric analysis of immunoblots. ∗ p < 0.05 and ∗∗ p < 0.01 by one-way ANOVA ( C ). IFNLR1 protein stability was analyzed by V5 immunoblotting. D and E , HEK-293T cells were transfected with V5-tagged IFNLR1 or V5-tagged IFNLR1 receptor plasmids with truncated C-terminal domains ( top schematic ) ( D ) or with V5-tagged IFNLR1 receptor plasmids with a series of 30 AA deletions ( E ). Twenty four hours later, cells were treated with DMSO or MG-132 (20 μM), and cell lysates were harvested for V5 immunoblotting. The data represent n = 3 separate experiments unless specified otherwise. CHX, cycloheximide; IFNLR1, interferon lambda receptor 1.

Article Snippet: Twenty four to forty eight hours after transfection, the cells were then treated with the protein synthesis inhibitor CHX (Cayman Chemical, 40–50 μg/ml) for the indicated times before collection.

Techniques: Transfection, Ubiquitin Proteomics, Plasmid Preparation, Western Blot